Results
| PMID | 17482186 |
| Gene Name | IL1R2 |
| Condition | Endometriosis |
| Association |
Associated |
| Sex | Female |
| Associated genes | IL-1 beta, IL-1RII |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2008 Jan;89(1):42-51. Epub 2007 May 4. Hou, Zhen| Zhou, Jing| Ma, Xiang| Fan, Lu| Liao, Lianming| Liu, Jiayin Center of Clinical Reproductive Medicine, Department of Obstetrics and Gynecology, First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China. OBJECTIVE: To determine the role of interleukin-1 receptor type II (IL-1RII) in the pathogenesis of endometriosis. DESIGN: Cultures of endometrial cells exposed to soluble IL-1RII or the recombinant adenovirus of IL-1RII (rAd-RII). SETTING: Gynecology clinic and human reproduction research laboratory. PATIENT(S): Women with endometriosis undergoing hysterectomy. INTERVENTION(S): Cell culture media were collected 12 hours after addition of soluble IL-1RII or infection of rAd-RII. MAIN OUTCOME MEASURE(S): The levels of IL-6 and IL-8 in the culture media were measured via enzyme-linked immunoabsorbent assay. Furthermore, proteins of the cells were collected for two-dimensional electrophoresis and the differential protein expression was identified by the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. RESULT(S): Addition of soluble IL-1RII (2.0 microg/mL) significantly inhibited IL-1 beta-induced IL-6 and IL-8 secretion by endometrial cells in vitro. Infection of endometrial cells with rAd-RII significantly decreased IL-1 beta-induced IL-8 secretion, compared with the PBS and rAd-LacZ controls but had no significant effect on IL-6 secretion. Proteins of the infected cells were collected for two-dimensional electrophoresis, and intensities of 62 spots were significantly increased or decreased when compared with those in the PBS group. Thirty-four proteins were identified by MALDI-TOF mass spectrometry. The majority of the identified proteins are related to cellular metabolism and proliferation. CONCLUSION(S): These results suggest that IL-1RII can neutralize IL-1 beta and counteract its effect on endometrial stromal cells, and may provide a new clinical strategy for the treatment of endometriosis. Mesh Terms: Adenoviridae/genetics| Adult| Cells, Cultured| Electrophoresis, Gel, Two-Dimensional| Endometriosis/genetics/*metabolism/pathology| Enzyme-Linked Immunosorbent Assay| Female| Genetic Vectors| Humans| Interleukin-1beta/metabolism| Interleukin-6/m |
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